Journal: Molecular Cancer Therapeutics

Article Title: Targeted Delivery of a Potent STING Agonist Payload via an Antibody–Drug Conjugate Drives Robust Antitumor Activity in Preclinical Models

doi: 10.1158/1535-7163.MCT-25-0108

Figure Lengend Snippet: Generation of a potent STING agonist ADC. A, Quantification of IFN reporter activity following treatment of THP1-Dual reporter cells with the indicated ADCs ( A ) or released payloads ( B ) for 48 or 24 hours, respectively. EC 50 values reported for the ADCs correspond to nmol/L of payload. Data are representative of two experiments. C–E, Quantification of RFP+ HT1080 tumor cell confluence of tumor cells cultured alone ( C ) or with PBMCs ( D and E ) following treatment with the indicated monoclonal antibody, ADC, or released payload for 4 days. Data are representative of 2–3 experiments. F, Mean tumor volume over time of human CD228-LL2 tumor–bearing C57BL/6 mice following treatment with a single 5 mg/kg intraperitoneal dose (arrow) of the indicated ADCs. Data represent a single in vivo experiment. Following a one-way ANOVA test, a Tukey post hoc multiple comparisons test was used to compare AUC.3 values for each treatment group: ***, P < 0.001; *, P < 0.05. Error bars depict SD.

Article Snippet: LL2 tumor cells (ATCC, #CRL-1642, RRID:CVCL_4358; purchased in 2018) were engineered using lentiviral transduction to express human CD228.

Techniques: Activity Assay, Cell Culture, In Vivo

Journal: Molecular Cancer Therapeutics

Article Title: Targeted Delivery of a Potent STING Agonist Payload via an Antibody–Drug Conjugate Drives Robust Antitumor Activity in Preclinical Models

doi: 10.1158/1535-7163.MCT-25-0108

Figure Lengend Snippet: Tumor-targeted ncSTING ADCs drive antitumor activity across multiple tumor models with multiple tumor antigens. Mean tumor volume over time of ( A ) human CD228-LL2 tumor–bearing C57BL/6 mice, ( B ) murine B7-H4-EMT6 tumor–bearing C57BL/6 mice, ( C ) murine B7-H4-Renca tumor–bearing Balb/c mice, and ( D ) murine IB6-CT26 tumor–bearing Balb/c mice following treatment with three weekly intraperitoneal doses (arrows) of the indicated ADCs. Data in each panel represent a single in vivo experiment. Following a one-way ANOVA test, a Tukey post hoc multiple comparisons test was used to compare each treatment group: ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05. Error bars depict SD.

Article Snippet: LL2 tumor cells (ATCC, #CRL-1642, RRID:CVCL_4358; purchased in 2018) were engineered using lentiviral transduction to express human CD228.

Techniques: Activity Assay, In Vivo

Journal: Molecular Cancer Therapeutics

Article Title: Targeted Delivery of a Potent STING Agonist Payload via an Antibody–Drug Conjugate Drives Robust Antitumor Activity in Preclinical Models

doi: 10.1158/1535-7163.MCT-25-0108

Figure Lengend Snippet: Tumor-targeted ncSTING ADCs with a WT Fc backbone drive enhanced antitumor activity in some, but not all, tumor models. Mean tumor volume over time of ( A ) human CD228-LL2 tumor–bearing C57BL/6 mice, ( B ) murine B7-H4-EMT6 tumor–bearing C57BL/6 mice, ( C ) murine B7-H4-Renca tumor–bearing Balb/c mice, ( D ) murine IB6-CT26 tumor–bearing Balb/c mice, ( E ) MC38 tumor–bearing C57BL/6 mice, and ( F ) MC38 tumor–bearing C57BL/6 WT and STING-deficient mice following treatment with the three weekly intraperitoneal doses (arrows) or a single dose (denoted by “x1”) of the indicated ADCs. Data in each panel represent a single in vivo experiment. Following a one-way ANOVA test, a Tukey post hoc multiple comparisons test was used to compare AUC.3 values for each treatment group: ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05. Error bars depict SD.

Article Snippet: LL2 tumor cells (ATCC, #CRL-1642, RRID:CVCL_4358; purchased in 2018) were engineered using lentiviral transduction to express human CD228.

Techniques: Activity Assay, In Vivo

Journal: Developmental cell

Article Title: Single Cell Profiling Reveals Sex, Lineage and Regional Diversity in the Mouse Kidney

doi: 10.1016/j.devcel.2019.10.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: QUANTIFICATION AND STATISTICAL ANALYSIS Single Cell RNASeq Data Analysis Principle component analysis and identification of variably expressed genes were carried out using the R packages Seurat (v 2.3.4) ( Butler et al., 2018 ), ggplot2 (v. 3.0), Matrix (v.1.2–14) and dplyr (v 0.7.5) in R Studio.

Techniques: In Situ Hybridization, Recombinant, Multiplex Assay, RNAscope, RNA Sequencing, Control, Software, Real-time Polymerase Chain Reaction, Microscopy, Flow Cytometry